perm kit (Multi Sciences (Lianke) Biotech Co Ltd)

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Multi Sciences (Lianke) Biotech Co Ltd perm kit
Perm Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fix+perm+kit/Fix+%26+Perm+Kit/10__34133_slash_bmr__0370-141-21-23
Average 95 stars, based on 146 article reviews

perm kit - by Bioz Stars, 2026-11

95/100 stars

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Staining:

Article Title: Leflunomide-Mediated Immunomodulation Inhibits Lesion Progression in a Vitiligo Mouse Model
Article Snippet: .. After surface staining, cells were fixed and permeabilized using the Fix/Perm Kit (Multi Sciences, CS1002) following the manufacturer’s guidelines. .. Intracellular IFN-γ was detected with PE-Cy7-conjugated anti-IFN-γ (1:50, F21IFNG05, Multi Sciences) in perm/wash buffer for 1 h at 25 °C.

Article Title: Leflunomide-Mediated Immunomodulation Inhibits Lesion Progression in a Vitiligo Mouse Model.
Article Snippet: .. After surface staining, cells were fixed and permeabilized using the Fix/Perm Kit (Multi Sciences, CS1002) following the manufacturer’s guidelines. .. Intracellular IFN-γ was detected with PE-Cy7-conjugated anti-IFN-γ (1:50, F21IFNG05, Multi Sciences) in perm/wash buffer for 1 h at 25 ◦C.

Centrifugation:

Article Title: Supporting Information for Restoring neuronal iron homeostasis revitalizes neurogenesis after spinal cord injury
Article Snippet: .. After centrifugation at 300 g for 5 min, cells were incubated with antibodies for 15 min at 25 °C and fixed with MEDIUM A of the fix & perm kit (Multi Sciences, China) for 5 min. After centrifugation at 300 g for 5 min, precipitation was resuspended by MEDIUM B of the kit and incubated with intracellular antibodies for 15 min, followed by washing 3 times. .. Cells were analyzed by flow cytometry (BD AccuriTM C6 cytometry, USA).

Incubation:

Article Title: Breaking the psoriasis pathological signaling cycle: A novel nanomedicine strategy targeting metabolism and oxidative stress.
Article Snippet: .. After washing with 5 % PBS, the cells were treated with FIX & PERM Kit (Multi sciences) and then incubated with APC-labeled anti-mouse IL-17A Antibody (506915) for another 20 min. ..

Article Title: Breaking the psoriasis pathological signaling cycle: A novel nanomedicine strategy targeting metabolism and oxidative stress
Article Snippet: The cells were incubated with the following antibodies for 20 min at room temperature in the absence of light: FITC-labeled anti-mouse CD3 Antibody (100204), PE/Cyanine7-labeled anti-mouse CD4 Antibody (100422), and PE-labeled anti-mouse CD69 Antibody (104508). .. After washing with 5 % PBS, the cells were treated with FIX & PERM Kit (Multi sciences) and then incubated with APC-labeled anti-mouse IL-17A Antibody (506915) for another 20 min. ..

other:

Article Title: Synergistic immune protection of exosomal T-cell epitope vaccine and antibody-inducing vaccine against SARS-CoV-2 in highly humanized mice
Article Snippet: Then, the cells were harvested, washed, blocked with anti-mouse CD16/CD32 (Miltenyi Biotec, 130-092-575) for 20 minutes at 4°C, and stained with FITC-anti-mouse CD3 (eBioscience, 11-0031-85, San Diego, CA, USA) and PE-anti-mouse CD4 antibodies (eBioscience, 12-0041-83) or PE-anti-mouse CD8a antibodies (eBioscience, 12-0081-83) for 30 minutes at 4°C.

Article Title: Simultaneous targeting of multiple etiological using a nanosized strategy for psoriasis management
Article Snippet: The cells were incubated with FITC anti-mouse CD3 (100204), RerCP/Cyanine5.5 anti-mouse CD4 (100433), PE anti-mouse CD69 (104508), and APC anti-mouse IL-17A (506915) antibody fluorescent following the standard protocols and assayed with a flow cytometer (Beckman Coulter, USA).

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